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Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing.
Transfer RNAs (tRNAs) play a central role in protein translation. Studying them has been difficult in part because a simple method to simultaneously quantify their abundance and chemical modifications is lacking. Here we introduce Nano-tRNAseq, a nanopore-based approach to sequence native tRNA populations that provides quantitative estimates of both...
Published: 9/29/2023
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Inventor(s): Eva Novoa, Morghan Lucas, Leszek Pryszcz, Rebeca Medina Azmecua
Keywords(s): Algorithms, Bioinformatics, Biomarkers, Diagnostics, Liquid Biopsy, Oncology
Category(s): Methodology, Software & Algorithms
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ELM-seq: A novel reporter system to optimize gene expression in vivo in an unbiased and comprehensive manner
Expression level monitoring by DNA methylation and sequencing (ELM-seq) allows optimizing sequence determinants of gene expression and translation comprehensively and more reliably than classical methods.The method can be of special relevance for bioengineering and industrial biotechnology, for the biosynthesis of useful molecules such as drugs, enzymes,...
Published: 2/19/2019
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Inventor(s): Eva Yus, Jae-Seong Yang, Luis Serrano
Keywords(s): Bioengineering, Bioinformatics, Biologics, Biomarkers, Biotechnology, Gene Regulation, Next Generation Sequencing, Protein Expression / Production, Protein Solubility, Vaccines
Category(s): Biotechnology, Methodology
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Novel strategy to find and eliminate latent HIV
The technology maps insert-specific expression across thousands of integrated HIV proviruses in the whole human genome.The technology can be used to identify the best (mix of) latency-reversing agents that can reactivate all latent HIV viruses which escape from antiretroviral therapies, making them susceptible to destruction.Technology:After integration...
Published: 2/19/2019
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Inventor(s): Guillaume Filion, Heng_chang Chen
Keywords(s): Cell-based Assay, Gene Regulation, Infectious Diseases
Category(s): Therapeutics (Biologics & Compounds), Drug Targets & Mechanisms, Methodology
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Method to identify new cancer drug targets in spliceosomal components
A new method and tools to map functional interactions of spliceosomal components accurately captures known, and identify new physical and functional associations important for health and disease.The method can be used to infer targets for alternative splicing regulation, and to study the effects on splicing of compounds directed to altered, or disease-associated...
Published: 2/19/2019
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Inventor(s): Juan Valcarcel, Juan Tejedor Vaquero, Panagiotis Papasaikas
Keywords(s): Algorithms, Bioinformatics, Genomics, Oncology
Category(s): Methodology, General Biology & Chemistry, Software & Algorithms
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Animal-free assessment of cell stemness
A method based on nucleosome detection by high resolution microscopy reliably assesses pluripotency much faster than the current gold standard without the use of animals.It can be used to identify the pluripotent potential of stem and iPS cells.Technology:Stem cell research has grown significantly over the last years and the assessment of stemness will...
Published: 11/12/2020
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Inventor(s): Maria Pia Cosma, Maria Aurelia Ricci
Keywords(s): Cell Therapy, Cell-based Assay, Stem Cells, Super-Resolution Nanoscopy, Tissue Engineering, Tissue Regeneration
Category(s): Methodology
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